LABORATORY FOR BIOCHEMISTRY OF STEROIDS AND ISOPRENOIDS

LAB1 at Institute of Biochemsitry, Faculty of Medicine, University of Ljubljana.

CURRENT LAB MEMBERS

Principal Investigators:

Marija Žakelj-Mavrič, Prof. of Biochemistry - Mail to: marija.zakelj-mavric[a]mf.uni-lj.si

Ana Plemenitaš, Prof. of Biochemistry - Mail to: ana.plemenitas[a]mf.uni-lj.si

Tea Lanišnik Rižner, Associate Prof. of Biochemistry - Mail to: tea.lanisnik-rizner[a]mf.uni-lj.si

Investigators:

Metka Lenassi - Mail to: metka.lenassi[a]mf.uni-lj.si

Neli Hevir - Mail to: neli.hevir[a]mf.uni-lj.si

Mojca Brunskole - Mail to: mojca.brunskole[a]mf.uni-lj.si

Ph.D. Students:

Natasa Beranic - Mail to: natasa.beranic[a]mf.uni-lj.si

Masa Sinreih - Mail to: masa.sinreih[a]mf.uni-lj.si

Martin Fettich - Mail to: martin.fettich[a]mf.uni-lj.si

Tilen Konte - Mail to: tilen.konte[a]mf.uni-lj.si

Lab Technician:

Milena Marušič - Mail to: milena.marusic[a]mf.uni-lj.si

Principal Funding:

Ministry of Education, Science and Sport of Slovenia:

P1-0170: Molecular mechanism of regulation of cellular processes in correlation with human disease
L3-6226: Endometriosis and Endometrial Cancer: Preparation of in vitro Model and Identification of Novel Drug Targets

Collaborations:

Prof. Jerzy Adamski, GSF-National Research Center for Environment and Health, Neuherberg, Germany
Prof. H.E. Gottlieb, Bar-Ilan University, Department of Chemistry, Israel
Prof. Jasna Peter-Katalinić, University of Muenster, Institute for Medical Physics and Biophysics, Germany
Prof. Trevor M. Penning, University of Pennsylvania, School of Medicine, Philadelphia, USA

Recent Selected Publications:

KRISTAN, K., LANIŠNIK RIŽNER, T., STOJAN, J., GERBER, J.K., KREMMER, E. and ADAMSKI, J. Significance of individual amino acid residues for coenzyme and substrate specificity of 17beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus. Chem.-Biol. Interact. 2003, 143-144: 493-501.

LANIŠNIK RIŽNER, T., LIN, H.K., PEEHL, D.M., STECKELBROECK, S, BAUMAN, D.R. and PENNING, T.M. Role of human type 3 3alpha -hydroxysteroid dehydrogenase (AKR1C2) in androgen metabolism of prostate cells. Endocrinology, 2003, 144: 2922-2932.

LANIŠNIK RIŽNER, T. and WHEELER, M. Melanin biosynthesis in the fungus Curvularia lunata (teleomorph Cochliobolus lunatus). Can. J. Microbiol. 2003, 49: 110-119.

LENASSI M., CAGNEY G., LIAO M., VAUPOTIC T., BARTHOLOMEEUSEN K., CHENG Y., KROGAN NJ., PLEMENITAS A., PETERLIN B.M. 2010. HIV Nef is secreted in exosomes and triggers apoptosis in bystander CD4+ T cells. Traffic. 11(1):110-22.

PLEMENITAS A., VAUPOTIC T., LENASSI M., KOGEJ T., GUNDE-CIMERMAN N. 2008. Adaptation of extremely halotolerant black yeast Hortaea werneckii to increased osmolarity: a molecular perspective at a glance. Stud Mycol. 61:67-75.

VAUPOTIC T., PLEMENITAS A. 2007. Differential gene expression and Hog1 interaction with osmoresponsive genes in the extremely halotolerant black yeast Hortaea werneckii. BMC Genomics.16;8:280.

LENASSI M., PLEMENITAS A. 2007. Novel group VII histidine kinase HwHhk7B from the halophilic fungi Hortaea werneckii has a putative role in osmosensing. Current Genetics. 51(6):393-405.

LENASSI M., VAUPOTIC T., GUNDE-CIMERMAN N., PLEMENITAS A. 2007. The MAP kinase HwHog1 from the halophilic black yeast Hortaea werneckii: coping with stresses in solar salterns. Saline Systems. 9;3:3.

VAUPOTIC T., GUNDE-CIMERMAN N., PLEMENITAS A. 2007. Novel 3'-phosphoadenosine-5'-phosphatases from extremely halotolerant Hortaea werneckii reveal insight into molecular determinants of salt tolerance of black yeasts. Fungal Genet Biol. 44(11):1109-22.

LANIŠNIK RIŽNER, T., STOJAN, J. and ADAMSKI, J. Searching for the physiological function of 17beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus: studies of substrate specificity and expression analysis. Mol. Cell. Endocrinol. 2001, 171: 193-198.

LANIŠNIK-RIŽNER T., ADAMSKI J., ŽAKELJ-MAVRIČ M. Expression of 17beta-hydroxysteroid dehydrogenases in mesophilic and extremophilic yeast. Steroids 2001, 66 (1), 49-54.

LANIŠNIK RIŽNER, T., ADAMSKI, J. and STOJAN, J. 17beta-hydroxysteroid dehydrogenase from Cochliobolus lunatus – model structure and substrate specificity. Arch. Biochem. Biophys. 2000, 384: 255-262.

LANIŠNIK-RIŽNER T., ADAMSKI J., ŽAKELJ-MAVRIČ M. Characterization of fungal 17beta-hydroxysteroid dehydrogenyses. Comp. Biochem. Physiol- Part B Biochem. Mol. Biol. 2000, 127 (1), 53-63.

ZORKO M., GOTTLIEB H.E., ŽAKELJ-MAVRIČ M. Pluripotency of 17beta hydroxysteroid dehydrogenase from the filamentous fungus Cochliobolus lunatus, Steroids 2000, 65, 46-53

LANIŠNIK RIŽNER, T., MOELLER, G., THOLE, H.H., ŽAKELJ-MAVRIČ, M. and ADAMSKI, J. A novel 17beta-hydroxysteroid dehydrogenase in the fungus Cochliobolus lunatus: new insights into the evolution of steroid hormone signalling. Biochem. J., 1999, 337: 425-431.

PLEMENITAŠ A., KASTELIC-SUHADOLC T., ŽIGON D., ŽAKELJ-MAVRIČ M. Steroidogenesis in the fungus Pleurotus ostreatus. Comp. Biochem. Physiol. Part B Biochem. Mol. Biol. 1999, 123 (2), 175-9.

Contact Information

Institute of Biochemistry
Faculty of Medicine
University of Ljubljana
Vrazov trg 2
SI-1000 Ljubljana
Slovenia
phone: +386-1-543-7652
fax: +386-1-543-7641